방사선에 조사된 인체세포주 (A431)에서 형태학적 분석법과 생화학적 분석법을 이용한 방사선 유도 세포고사에 관한 연구 ( Studies on the Radiation Induced Apoptosis by Morphological and Biochemical Analysis in A431 Cells ) () |
Author |
최근희(Keun Hee Choi),범희승(Hee Seung Bom),김지열(Ji Yeul Kim), |
|
Affiliation |
|
Abstract |
Purpose: We performed this study to evaluate the
process of radiation induced apoptosis in A431 skin
epithelial cancer cell line. Materials and Methods: Low
to high dose radiation (0, 2, 5, 10, 25 Gy) was given
to A431 cells by Cs-137 cell irradiator. Apoptosis was
evaluated by cell morphology, dye exclusion test, and
DNA laddering. Results: Cell viability decreased as the
radiation dose increased. Number of apoptotic bodies
increased as radiation dose increased. It increased
most significantly at 12 hours after irradiation.
Lactate dehydrogenase activity in culture medium
increased according to radiation dose and time after
irradiation. Conclusion: Radiation-induced apoptosis
which was the main course of cell death in A431 cells
could be analyzed quantitatively by counting apoptotic
bodies under microscope. Apoptosis increased as
radiation dose increased. |
Keyword |
Radiation-induced apoptosis, A431 cell line, Apoptotic body, DNA ladder |
Full text Article |
28502407.pdf
|
|
|