Author |
이태섭(Tae Sup Lee),서용섭(Yong Sup Suh),최창운(Chang Woon Choi),우광선(N/A),정위섭(Wee Sup Chung),임수정(Soo Jung Lim),임상무(Sang Moo Lim),오옥두(Ok Doo Awh), |
Abstract |
Purpose: 123I-labeled fatty acids have been used in the
evaluation of regional myocardial energy metabolism.
This study aimed to evaluate the usefulness of 123I-
BMIPP as a liposarcorna-imaging agent. Materials and
Methods: We compared in vitro uptakes between
liposarcoma(SW872) and glioma(9L) cell lines, and
examined biodistribution and in vivo images of 123I-
BMIPP in liposarcoma-bearing nude mice. Cold-BMIPP was
labeled with 123I using Cu2+ as catalyst. After
purification by Sep-pak, radiochemical purity was
determined by TLC. We compared cellular uptake between
glioma and liposarcoma after incubation of 5, 10, 15,
30, 60, 120, and 180 mins with culture medium
containing I-BMIPP. The difference in biodistribution
was determined between non-feeding (water only) group
for 18 hr and feeding group in normal mice (n=6/group)
at 0.5, 2, and 24 hr. In liposarcoma-bearing nude mice
model, liposarcoma, SW872, cell lines were injected
subcutaneously into the left thigh of nude mice. The
biodistribution of 123I-BMIPP was evaluated at 0.5, 2,
and 24 hr (n=5 / group) and in vivo image of 123I-BMIPP
was obtained with gamma camera at 2 and 24 hr in
liposarcorna-bearing nude mice. Results: Radiolabeling
yield and radiochemical purity were 95% and above 99%,
respectively. SW872 cell line showed more increased
uptake than 9L with 1.5 times at 180 mins. The
clearance of 'I-BMIPP in various tissues was more
delayed in the non-feeding group than in the feeding
group, especially at delayed time (24 hr) in normal
mice, and the major excreting organ was the
gastrointestinal tract. In liposarcoma-bearing nude
mice, tumor/blood ratio of 123I-BMIPP was 0.94, 0.75,
and 1.38 and tumor/muscle ratio was 0.66, 1.53, and
1.11 at 0.5, 2, and 24hr, respectively. 123I-BMIPP was
selectively localized in liposarcoma at 24 hr image.
Conclusions: These results suggest that 123I-BMIPP can
be used as a liposarcoma-imaging agent. (Korean J Nucl
Med 200135:324-333) |