Abstract |
Technetium-99 m-hexamethylpropyleneamine oxime (99mTc-
HMPAO) is a neutral-lipophilic chelate which is used
for scanning cerebral blood flow. The labeling
efftciencies of 99mTc-HM-PAO is known to be sensitive
to the amount of pertechnetate added and the quality of
the pertechnetate. Because of these factors, the
manufacture recornmends that HM-PAO kits be
reconstituted with a maximum of 30 mCi pertee.hnetate
which was eluted <4 hr earlier from a generator which
had been eluted <24 hr previously. So we measured the
labelling efficiencies and the decomposition rate
constant according to the amount of pertechnetate
added, the volume of pertechnette added, and generator
in-growth time. We used the 3-system chromatagraphic
rnethods (paper & ITLC-SG chrornatography) which
analyzed the labelling efficiencies of the 99mTc-HM-
PAO. There was no significant difference in labelling
efficiencies between variable pertechnetate acitvities
added. (39.9 +- 4.9mCi: 87.8 +- 5.1 (%), 60.8 +-
5.0mCi: 90.7 +- 2.2(%), 79.0 +- 6.0mCi: 86.8 +- 3.9
(%), 106.6 +- 11.6mCi: 87.7 +- 1.2 (%), p>0.05) No
significant difference in labelling efficiencies were
found between pertechnetate of 4 ml and 5 ml. (4 ml:
89.1+3.2 (%), 5 ml: 87.3+4.0 (%), p> 0.05) There was no
difference between 1-6 and 10 +-48 hr of generator in-
growth time. (1-6 hr: 87 8+ 4.0 (%), 10 +- 48 hr:
89.6+1.6 (%), p>0.05) The mean value of decompositian
rate constant was 0.196+ 0.097 (hr-1), and there were
no differerce according to the amount of pertecnetate
added and the volume of pertecnetate added, (39.9+4.9
mCi: 0.208+0.059 (hr-1), 60.8 +- 5.0 mCi: 0.191 +-
0.100 (hr-1) 79.06.0mCi: 0.192 +- 0.118 (hr-1 ), 106.6
+- 11.6mCi: 0.212 +- 0.030 (hr-1), p>0.05, 4ml:
0.200+0.074 (hr-1), 5mi: 0.193 +- 0.115 (hr-1), p>0.05)
In the case of using the first eluate, the labeliing
efficiency of 99mTc-HM-PAO was 82.1%..
These data suggest that there were no significant
aiteration in labelling efficiency of 99m Tc-HM- PAO
aecording to the considerable range of pertechnetate
activities and volume added, and generator in-growth
time. Also, it was shown that one vial of HM-PAO kit
supplied the Tc-HM-PAO which was used for 3-4 patients. |