To detect ischemic tissue in experimental model of cerebral ischemia made by middle cerebral artery(MCA)- occlusion, we acquired triple image of Tc-99m- glucarate, [18F]fluoro-deoxyglucose (FDG), and 2,3,5- triphenyltetrazolium (TTC) staining. We made cerebral infarction either with reperfusion (after occlusion of 2 hours) or without reperfusion in 10 Sprague-Dawley rats by inserting thread to MCA through internal carotid artery. After 22 hours, we injected 740 MBq of Tc-99m-glucarate and 55.5 MBq of [18F]FDG through tail vein. Each 1 mm slice of rat brains was frozen and exposed to imaging plate for 20 minutes in freezer to get an [18F]FDG image. After 20 hours enough to fade radioactivity of [18F]FDG, the slices were again imaged by BAS1500 for Tc-99m-glucarate uptake¡¤Finally, these braim tissues were stained with TTC. semi- quantitative visual analysis was done by grading 0 to 3 points according to the degree of uptakes(Tc-99m- glucarate) and decreased uptakes([18F]FDG and TTC). Ten rats survived with neurologic symptoms. TTC staining confirmed the development of infarction. The size of the infarction was relatively larger in the group without reperfusion. [18F]FDG images were similar to TTC-stained images. However, we found regions with intermediate uptake which were not stained with TTC. We found regions with intermediate [18F]FDG uptake where TTC staining was normal. Tc-99m-glucarate uptake was found only in TTC non-stained region. In the TTC stained regions, there were no uptake of Tc-99m- glucarate. We could not find clear relation between Tc- 99m- glucarate uptake with [18F]FDG uptake. This was partly because percent uptake of Tc-99m- glucarate was so small (less than 1 percent of injected dose) and because there were quite heterogeneity of patterns of [18F]FDG uptake and TTC. With these findings, we could conclude that Tc-99m-glucarate were taken up only in part of ischemic tissues which were proven to be nonviable. The establishment of MCA-occluded rat model with or without reperfusion and triple imaging for Tc- 99m, 18F and TTC helped the characterzation of Tc-99m- glucarate uptakes. Further work is needed to clarify the meaning of diversities of [18F]FDG and TTC and their relation with Tc-99m-glucarate.